FAM-FMK - Caspase 1 Detection by Cell Technology
Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. Cell permeablity permits direct visualization of cytosolic apoptotic events. Apoptotic cell population does not diminish over time. Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure. Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments. Yields both quantitative and qualitative results. Gives strong signal with little background noise.
FAM-FMK - Caspase 1 Detection by Cell Technology
APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.
Caspase-1
Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping
United States
FAM600-1: 25 Tests
Lyophilized FAM Labeled Peptide (FAM-YVAD-FMK), 1 vial (Part # 8005)
10X Apoptosis Wash Buffer, 15 mL (Part # 3028)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
FAM600-2: 100 TestsLyophilized FAM Labeled Peptide (FAM-YVAD-FMK), 4 vials (Part # 8005)
10X Apoptosis Wash Buffer, 60 mL (Part # 3026)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
FAM600-2: 100 Tests
Product Specific References
| PMID | Publication |
| 36794663 | Lv, J., et al. 2023. Bone marrow mesenchymal stem cell-derived exosomal miR-30e-5p ameliorates high-glucose induced renal proximal tubular cell pyroptosis by inhibiting ELAVL1. Ren Fail, . |
| 37161797 | He, W., et al. 2023. LOXL1-AS1 Aggravates Myocardial Ischemia/Reperfusion Injury Through the miR-761/PTEN Axis. Korean Circ J, 387-403. |
| 36752740 | Liu, D., et al. 2023. Ginsenoside Rg3 Ameliorates DSS-Induced Colitis by Inhibiting NLRP3 Inflammasome Activation and Regulating Microbial Homeostasis. Agric Food Chem, . |
| 26709768 | Li, Y. F., et al. 2016. Caspase-1 mediates hyperlipidemia-weakened progenitor cell vessel repair. Front Biosci (Landmark Ed), 178-91. |
| 16420693 | Jiang, W., et al. 2006. The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells. Mol Cancer, 4. |
| 26037927 | Lopez-Pastrana, J., et al. 2015. Inhibition of Caspase-1 Activation in Endothelial Cells Improves Angiogenesis: A NOVEL THERAPEUTIC POTENTIAL FOR ISCHEMIA. J Biol Chem, 17485-94. |
| 31315440 | Kearns, A. C., et al. 2019. Caspase-1 Activation Is Related With HIV-Associated Atherosclerosis in an HIV Transgenic Mouse Model and HIV Patient Cohort. Arterioscler Thromb Vasc Biol, 1762-1775. |