FAM-FMK - Caspase 8 Detection by Cell Technology
Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. Cell permeablity permits direct visualization of cytosolic apoptotic events. Apoptotic cell population does not diminish over time. Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure. Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments. Yields both quantitative and qualitative results. Gives strong signal with little background noise.
FAM-FMK - Caspase 8 Detection by Cell Technology
APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.
Caspase-8
Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping
United States
FAM300-1: 25 Tests
Lyophilized FAM Labeled Peptide (FAM-LETD-FMK), 1 vial (Part 8004)
10X Apoptosis Wash Buffer, 15 mL (Part # 3028)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
FAM300-2: 100 TestsLyophilized FAM Labeled Peptide (FAM-LETD-FMK), 4 vials (Part 8004)
10X Apoptosis Wash Buffer, 60 mL (Part # 3026)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
FAM300-2: 100 Tests
Product Specific References
| PMID | Publication |
| 16420693 | Jiang, W., et al. 2006. The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells. Mol Cancer, 4. |
| 28679299 | Xu, W., et al. 2018. Arsenic trioxide decreases the amount and inhibits the function of regulatory T cells, which may contribute to its efficacy in the treatment of acute promyelocytic leukemia. Leuk Lymphoma, 650-659. |
| 23732220 | Huang, H., et al. 2013. Naringin inhibits high glucose-induced cardiomyocyte apoptosis by attenuating mitochondrial dysfunction and modulating the activation of the p38 signaling pathway. Int J Mol Med, 396-402. |
| 16179598 | Zeng, H., et al. 2006. Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. Am J Physiol Gastrointest Liver Physiol, G96-G108. |
| 23077597 | Lv, X., et al. 2012. Berberine inhibits doxorubicin-triggered cardiomyocyte apoptosis via attenuating mitochondrial dysfunction and increasing Bcl-2 expression. PLoS One, e47351. |
| 12855622 | Wall, L., et al. 2003. IFN-gamma induces apoptosis in ovarian cancer cells in vivo and in vitro. Clin Cancer Res, 2487-96. |
| 21406729 | Eberhard, Y., et al. 2011. Inhibition of SREBP1 sensitizes cells to death ligands. Oncotarget, 186-96. |