FLICA 660 Caspase-3/7 Assay Kit

from ImmunoChemistry Technologies Product Manual Safety Data Sheet
6 Citations
Detect caspase 3 and 7 activity with the FLICA 660 Caspase 3/7 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe 660-DEVD-FMK to label active caspase 3 and 7 enzyme in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.

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FLICA 660 Caspase-3/7 Assay Kit
FLICA 660 Caspase-3/7 Assay Kit
FLICA 660 Caspase-3/7 Assay Kit
FLICA 660 Caspase-3/7 Assay Kit
FLICA 660 Caspase-3/7 Assay Kit

FLICA 660 Caspase-3/7 Assay Kit
SKU: 9125

Size: 25-50 Tests
Price:
$248

Bulk Order FLICA 660 Caspase-3/7 Assay Kit

Caspases play important roles in apoptosis and inflammation. ICT’s FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells. The FLICA 660 Caspase 3/7 probe allows researchers to assess caspase 3 & 7 activation. The FLICA reagent 660-DEVD-FMK enters each cell and irreversibly binds to activated caspase 3 and 7. Because the 660-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound 660-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining far red fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by fluorescence microscopy or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative. Unfixed samples may also be analyzed with Hoechst stain to detect changes in nuclear morphology.
660-DEVD-FMK
Caspase 3 and 7
660 nm / 690 nm
Flow cytometry, Fluorescence microscope
Cell culture
2-8°C
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United States
  1. Prepare samples and controls
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH2O.
  3. Reconstitute FLICA with 50 µL DMSO.
  4. Dilute FLICA 1:5 by adding 200 µL PBS.
  5. Add diluted FLICA to each sample at 1:30-1:60 (e.g. spike at 1:30 by adding 10 µL to 290 µL cultured cells).
  6. Incubate approximately 1 hour.
  7. Wash and spin cells three times.
  8. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody.
  9. If desired, fix cells.
  10. Analyze with a fluorescence microscope or flow cytometer. FLICA 660 excites at 660 nm and emits at 680-690 nm.
  • FLICA Caspase-3/7 Inhibitor Reagent (660-DEVD-FMK), 1 vial, #6344
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Kit Manual

    • Kodali, V;Kim, KS;Roberts, JR;Bowers, L;Wolfarth, MG;Hubczak, J;Xin, X;Eye, T;Friend, S;Stefaniak, AB;Leonard, SS;Jakubinek, M;Erdely, A. Influence of Impurities from Manufacturing Process on the Toxicity Profile of Boron Nitride Nanotubes. Small (Weinheim an der Bergstrasse, Germany). 2022 November 14; doi: 10.1002/smll.202203259. Research Article

    Deng, N;Zuo, X;Lin, Q;Wang, T;Li, Y;Zhong, J;Ni, H;Chen, Q;Ding, X;Yu, H;Nie, H. Low-dose 5-fluorouracil ameliorates Th2 responses through the induction of apoptotic cell death of lung monocyte-derived dendritic cells in asthma. Biomedicine & pharmacotherapy. 2022 October 19; doi: 10.1016/j.biopha.2022.113875. Full Text

    Su, M;Chen, C;Li, S;Li, M;Zeng, Z;Zhang, Y;Xia, L;Li, X;Zheng, D;Lin, Q;Fan, X;Wen, Y;Liu, Y;Chen, F;Luo, W;Bu, Y;Qin, J;Guo, M;Qiu, M;Sun, L;Liu, R;Wang, P;Hwa, J;Tang, WH. Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis. Nature cardiovascular research. 2022 August 4; doi: 10.1038/s44161-022-00108-7. Citation

    McLemore, AF; Hou, HA; Meyer, BS; Lam, NB; Ward, GA; Aldrich, AL; Rodrigues, MA; Vedder, A; Zhang, L; Padron, E; Vincelette, ND; Sallman, DA; Abdel-Wahab, O; List, AF; McGraw, KL. Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS-STING-NLRP3 axis in Myelodysplastic syndromes. JCI Insight. 2022 July 5; doi: 10.1172/jci.insight.159430. Article

    Palacios-Macapagal D, Connor J, Mustelin T, Ramalingam TR, Wynn TA, and Davidson TS. Cutting Edge: Eosinophils Undergo Caspase-1-Mediated Pyroptosis in Response to Necrotic Liver Cells. J. Immunol. 2017. Aug 1;199(3):847-853. doi: 10.4049/jimmunol.1601162. Epub 2017 Jun 26. Abstract.

    Peng R, Zhang W, Zuo Z, Shan Y, Liu X, Tang Y, Yu Z, Wang L, Cong Y. Dimethyl sulfoxide, a potent oral radioprotective agent, confers radioprotection of hematopoietic stem and progenitor cells independent of apoptosis. Free Radic Biol Med. 2020 Mar 25;153:1-11. doi: 10.1016/j.freeradbiomed.2020.03.021. [Epub ahead of print]. Full Text

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