By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.
General-Purpose Sample Diluent is formulated for diluting goat, rabbit, mouse, and human sera, as well as cell culture media and mouse ascites fluid. ESD100 can also be used for dilution of mammalian and chicken serum samples in antigen-down ELISA formats. Hyperimmunized serum samples contain nonspecific IgG antibody that may interfere with the titration. Proprietary additives have been incorporated into this diluent that minimize nonspecific binding interactions of the non specific IgG in the samples, thus decreasing background noise. Dilutions of the antigen standard for use in sandwich ELISA formats have also been carried out using this diluent. ESD100 contains a buffered BSA protein base to assure a constant pH and solute environment favorable to antibody-antigen interactions. It also contains an antimicrobial agent to provide a solution free of bacterial contamination.
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Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.