Hoechst 33342 Fluorescent Nucleic Acid Stain
Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pyknotic nuclei in apoptotic cells and for cell cycle studies.
Hoechst 33342 Fluorescent Nucleic Acid Stain
Hoechst 33342 is a popular cell-permeant, blue fluorescent nuclear stain. It is used to visualize the nuclei of living or fixed cells and tissues and is often used to distinguish condensed, pyknotic nuclei in apoptotic cells. Hoechst 33342 emits blue fluorescence when bound to double stranded DNA. It is slightly more membrane permeant than the Hoechst 33258 analog. Hoechst 33342 may be used to identify healthy or apoptotic nuclear morphology and for cell cycle studies.
350 nm / 480 nm
Flow cytometry, Fluorescence microscope
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
5.0 + 0.5
200 µg/mL
United States
- Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 µL Hoechst to 300 µL of cells. 2. Incubate 10-20 minutes at room temperature.
- Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
- Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
- When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.
Product Specific References
| PMID | Publication |
| 37425955 | de Sousa, F.T.G., et al. 2023. Yellow fever disease severity and endothelial dysfunction are associated with elevated serum levels of viral NS1 protein and syndecan-1. medRxiv : the preprint server for health sciences, . |
| 37543157 | Luscombe, V.B., et al. 2023. Kinetic insights into agonist-dependent signalling bias at the pro-inflammatory G-protein coupled receptor GPR84. European journal of pharmacology, 175960. |
| 37602284 | Ivanusic, D., et al. 2023. The large extracellular loop is important for recruiting CD63 to exosomes. microPublication biology, . |
| 37666180 | Ho, A., et al. 2023. IL-34 exacerbates pathogenic features of Alzheimer's disease and calvaria osteolysis in triple transgenic (3x-Tg) female mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 115435. |
| 36640308 | Schleinitz, A., et al. 2023. Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes. Cell reports, 111969. |
| 35237179 | Sato, R., et al. 2022. Extracellular Vesicles Derived From Murine Cementoblasts Possess the Potential to Increase Receptor Activator of Nuclear Factor-κB Ligand-Induced Osteoclastogenesis. Frontiers in Physiology, . |
| 35355981 | Lučiūnaitė, A., et al. 2022. Activation of NLRP3 Inflammasome by Virus-Like Particles of Human Polyomaviruses in Macrophages. Frontiers in immunology, 831815. |
| 35387562 | De Mazière, A., et al. 2022. An optimized protocol for immuno-electron microscopy of endogenous LC3. Autophagy, 44945. |
| 35429112 | Duarte, C., et al. 2022. Crosstalk between dihydroceramides produced by Porphyromonas gingivalis and host lysosomal cathepsin B in the promotion of osteoclastogenesis. Journal of cellular and molecular medicine, . |
| 35640691 | Ophélie, G., et al. 2022. Cellular and molecular mechanisms of NiONPs toxicity on eel hepatocytes HEPA-E1: An illustration of the impact of Ni release from mining activity in New Caledonia. Chemosphere, 135158. |
| 35644541 | Kiriya, M., et al. 2022. Thyroglobulin regulates the expression and localization of the novel iodide transporter solute carrier family 26 member 7 (SLC26A7) in thyrocytes. Endocrine journal, . |
| 35728871 | Chinn, H.K., et al. 2022. Hypoxia-inducible lentiviral gene expression in engineered human macrophages. Journal for immunotherapy of cancer, . |
| 35862783 | Lu, S.L., et al. 2022. VEGF-Mediated Augmentation of Autophagic and Lysosomal Activity in Endothelial Cells Defends against Intracellular Streptococcus pyogenes. mBio, e0123322. |
| 35658124 | Xia, M., et al. 2022. BCL10 Mutations Define Distinct Dependencies Guiding Precision Therapy for DLBCL. Cancer discovery, 1922-1941. |
| 36004615 | Buscetta, M., et al. 2022. Cigarette smoke promotes inflammasome-independent activation of caspase-1 and -4 leading to gasdermin D cleavage in human macrophages. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, e22525. |
| 36183103 | Schroeder-Castagno, M., et al. 2022. Impaired response of blood neutrophils to cell-death stimulus differentiates AQP4-IgG-seropositive NMOSD from MOGAD. Journal of neuroinflammation, 239. |
| 36359010 | Wu, Z., et al. 2022. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules, . |
| 36527745 | Konishi, H., et al. 2022. Osimertinib-tolerant lung cancer cells are susceptible to ferroptosis. Biochemical and biophysical research communications, 116-122. |