Quenched Cell permeable dye.
One Step, No wash Homogenous assay.
Adaptable to High throughput assay platforms.
Can monitor multiple time points to follow real time kinetics.
Non-destructive cell based assay allows monitoring of additional parameters.
Aminophenyl fluorescein (APF), developed by Tetsuo Nagano, is a general selective indicator for the
detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS
such as: singlet oxygen (O21), superoxide (O2– •), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl
peroxide (RO2•). APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (•OH),
Peroxynitrite: (ONOO-) and hypochlorite (-OCl) production in cells.
Fluorescence plate reader
2-8°C
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