Quenched Cell permeable dye. One Step, No wash Homogenous assay. Adaptable to High throughput assay platforms. Can monitor multiple time points to follow real time kinetics. Non-destructive cell based assay allows monitoring of additional parameters.
Aminophenyl fluorescein (APF), developed by Tetsuo Nagano, is a general selective indicator for the detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS such as: singlet oxygen (O21), superoxide (O2– •), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•). APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (•OH), Peroxynitrite: (ONOO-) and hypochlorite (-OCl) production in cells.
Fluorescence plate reader
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