Mitochondrial Membrane Potential Detection Kit

The kit uses a unique dye to detect the collapse of the mitochondrial membrane potential in apoptotic and necrotic cells. Results can be analyzed by flow cytometry, fluorescence microscopy or fluorescence plate reader.

SKU: JC100

Size: 100 Tests
Sale price$313.00

Mitochondrial Membrane Potential detection kit provides an early indication of the initiation of cellular apoptosis. This process is typically defined as the collapse in the electrochemical gradient across the mitochondrial membrane, as measured by the change in the membrane potential. The insertion and oligomerization of proapoptotic proteins BID, BAK, BAX or BAD create pores which could dissipate the trans-membrane potential and release cytochrome C into the cytoplasm.

The Mitochondrial Membrane Potential Detection Kit uses a unique cationic dye (5,5’,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimidazolylcarbocyanine iodide) to signal the loss of the mitochondrial membrane potential. In healthy cells, the dye stains the mitochondria bright red. The negative charge established by the intact mitochondrial membrane potential allows the lipophilic dye, bearing a delocalized positive charge, to enter the mitochondrial matrix where it accumulates. When the critical concentration is exceeded, J- aggregates form which become fluorescent red. In apoptotic cells, the mitochondrial membrane potential collapses, and the dye cannot accumulate within the mitochondria. In these cells the dye remains in the cytoplasm in a green fluorescent monomeric form. Apoptotic cells, showing primarily green fluorescence, are easily differentiated from healthy cells which show red and green fluorescence. The aggregate red form has absorption/emission maxima of 585/590 nm. The green monomeric form has absorption/ emission maxima of 510/527 nm. Both apoptotic and healthy cells can be visualized simultaneously by fluorescence microscopy using a wide band-pass filter suitable for detection of fluorescein and rhodamine emission spectra. The dye reagent is easy to use. Simply dilute the reagent in cell culture medium and add to the cells. After a 15 minute incubation, wash the cells and analyze by flow cytometry or fluorescence microscopy or fluorescence plate reader.

Flow Cytometry, Fluorescent plate reader or Fluorescent microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping
United States
  • Part# 4001: Lyophilized JC-1 Dye (Store at 2-8C)
  • Part# 3002: 10X Assay Buffer (Store at 2-8C)
  • Product Specific References

    PMID Publication
    36618917Zhang, H., et al. 2022. Red ginseng extract improves skeletal muscle energy metabolism and mitochondrial function in chronic fatigue mice. Front Pharmacol, 1077249.
    33432343Umbaugh, D.S., et al. 2021. Mitochondrial Membrane Potential Drives Early Change in Mitochondrial Morphology After Acetaminophen Exposure. Toxicol Sci, 186-195.
    30693839Lo, M.C., et al. 2018. Alpha-lipoic acid suppresses Nε-(carboxymethyl) lysine-induced epithelial mesenchymal transition in HK-2 human renal proximal tubule cells. Free Radic Res, 1387-1397.
    29764240Kuo, Y., et al. 2018. Ferrous glycinate regulates cell energy metabolism by restrictinghypoxia-induced factor-1α expression in human A549 cells. Free Radic Res, 1348-1358.
    26912141Nagai, K., et al. 2016. The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Biomed Res, 63-71.
    26902083Yao, J., et al. 2016. Assessment of the Cytotoxic and Apoptotic Eἀects of Chaetominine in a Human Leukemia Cell Line. Biomol Ther (Seoul), 147-55.
    25740140Mohidin, T.B., et al. 2015. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells . J Biosci, 41-51.
    25805179Perera, Y., et al. 2015. Pharmacologic inhibition of the CK2-mediated phosphorylation of B23/NPM in cancer cells selectively modulates genes related to protein synthesis, energetic metabolism, and ribosomal biogenesis. Mol Cell Biochem, 103-12.
    25891083Zheng, K.M., et al. 2015. Curcumin inhibits appoptosin-induced apoptosis via upregulating heme oxygenase-1 expression in SH-SY5Y cells. Acta Pharmacol Sin, 544-52.
    23098068Camgoz, A., et al. 2013. Mechanisms responsible for nilotinib resistance in human chronic myeloid leukemia cells and reversal of resistance . Leuk Lymphoma, 1279-87.
    23911596Lai, J.H., et al. 2013. Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1α. Neuro Oncol, 1330-41.
    23158585Selvaraj, V., et al. 2013. Selenium (sodium selenite) causes cytotoxicity and apoptotic mediated cell death in PLHC-1 fish cell line through DNA and mitochondrial membrane potential damage. Ecotoxicol Environ Saf, 80-8.
    22079007Magalhães, R., et al. 2012. Influence of cell culture configuration on the post-cryopreservation viability of primary rat hepatocytes . Biomaterials, 829-36.
    23052231Syed, I., et al. 2012. L- threo -C6-pyridinium-ceramide bromide, a novel cationic ceramide, induces NADPH oxidase activation, mitochondrial dysfunction and loss in cell viability in INS 832/13 β-cells. Cell Physiol Biochem, 1051-8.
    23023949Selvaraj, V., et al. 2012. Protective and antioxidant role of selenium on arsenic trioxide-induced oxidative stress and genotoxicity in the fish hepatoma cell line PLHC-1. Environ Toxicol Chem, 2861-9.
    21455605Gencer, E., et al. 2011. A novel mechanism of dasatinib-induced apoptosis in chronic myeloid leukemia; ceramide synthase and ceramide clearance genes . Ann Hematol, 1265-75.
    21483866Rizvi, F., et al. 2011. Mitochondrial dysfunction links ceramide activated HRK expression and cell death. PLoS One, e18137.
    21500096Kartal, M., et al. 2011. Resveratrol triggers apoptosis through regulating ceramide metabolizing genes in human K562 chronic myeloid leukemia cells . Nutr Cancer, 637-44.
    22331716Gucluler, G., et al. 2011. The roles of antiapoptotic sphingosine kinase-1 and glucosylceramide genes in drug induced cell death of MCF-7 breast cancer cells . J BUON, 646-51.
    21976958Liu, C., et al. 2011. SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells. Mol Vis, 2473-81.
    20307198Baran, Y., et al. 2010. Combination of fludarabine and imatinib induces apoptosis synergistically through loss of mitochondrial membrane potential and increases in caspase-3 enzyme activity in human K562 chronic myleloid leukemia cells. Cancer Invest, 623-8.
    20036686Sharma, H., et al. 2010. Organochlorine pesticides dieldrin and lindane induce cooperative toxicity in dopaminergic neurons: role of oxidative stress. Neurotoxicology, 215-22.
    20815788Minamiyama, Y., et al. 2010. Generation of reactive oxygen species in sperms of rats as an earlier marker for evaluating the toxicity of endocrine-disrupting chemicals. Free Radic Res, 1398-406.
    20616211Yan, H.M., et al. 2010. The oxygen tension modulates acetaminophen-induced mitochondrial oxidant stress and cell injury in cultured hepatocytes. Toxicol Sci, 515-23.
    19490758Gucluler, G., et al. 2009. Docetaxel enhances the cytotoxic effects of imatinib on Philadelphia positive human chronic myeloid leukemia cells . Hematology, 139-44.
    18711728Liu, J., et al. 2009. Effects of neuroglobin overexpression on mitochondrial function and oxidative stress following hypoxia/reoxygenation in cultured neurons. J Neurosci Res, 164-70.
    19226332Lang, M., et al. 2009. Epigallocatechin-gallate modulates chemotherapy-induced apoptosis in human cholangiocarcinoma cells. Liver Int, 670-7.
    18022342Lengwehasatit, I., et al. 2008. Involvement of B. pseudomallei RpoS in apoptotic cell death in mouse macrophages. Microb Pathog, 238-45.
    17936297Liu, Z.G., et al. 2007. Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol, 334-45.
    16896059Bajt, M., et al. 2006. Nuclear translocation of endonuclease G and apoptosis-inducing factor during acetaminophen-induced liver cell injury. Toxicol Sci, 217-25.
    16427301Panzarini, E., et al. 2006. Apoptosis induction and mitochondria alteration in human HeLa tumour cells by photoproducts of Rose Bengal acetate. J Photochem Photobiol B, 39-47.
    15782122Liu, Y., et al. 2005. Opposing effects of bovine papillomavirus type 1 E6 and E7 genes on Fas-mediated apoptosis. Oncogene, 3942-53.
    15936846Ling, X., et al. 2005. Differential expression of survivin-2B and survivin-DeltaEx3 is inversely associated with disease relapse and patient survival in non-small-cell lung cancer (NSCLC). Lung Cancer, 353-61.
    15500008Yount, G., et al. 2004. Berberine sensitizes human glioma cells, but not normal glial cells, to ionizing radiation in vitro . J Exp Ther Oncol, 137-43.
    14970181Lawson, B., et al. 2004. Deficiency of the cyclin kinase inhibitor p21(WAF-1/CIP-1) promotes apoptosis of activated/memory T cells and inhibits spontaneous systemic autoimmunity. J Exp Med, 547-57.

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