JC 1 MMP Detection by Cell Technology
Cell permeability allow direct measurement of apoptosis and mitochondrial potential in live cells. Incubate for 15 minutes, wash and measure. Add this reagent directly to live cells in your media of choice.
JC 1 MMP Detection by Cell Technology
Detection of the mitochondrial permeability transition event provides an early indication of the initiation of cellular apoptosis. This process is typically defined as a collapse in the electrochemical gradient across the mitochondrial membrane, as measured by the change in the membrane potential (YD). Loss of mitochondrial (YD) is indicative of apoptosis and can be detected by a unique fluorescent cationic dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl- benzamidazolocarbocyanin iodide, commonly known as JC-1. This dye has been incorporated into the user-friendly kit for the simple and reproducible detection of the membrane potential (YD) event in apoptotic cells. The kit has been formatted for use on Flow cytometers, Fluorescent plate readers and Fluorescent Microscopes
Flow Cytometry, Fluorescent plate reader or Fluorescent microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping
United States
Product Specific References
| PMID | Publication |
| 36618917 | Zhang, H., et al. 2022. Red ginseng extract improves skeletal muscle energy metabolism and mitochondrial function in chronic fatigue mice. Front Pharmacol, 1077249. |
| 33432343 | Umbaugh, D.S., et al. 2021. Mitochondrial Membrane Potential Drives Early Change in Mitochondrial Morphology After Acetaminophen Exposure. Toxicol Sci, 186-195. |
| 30693839 | Lo, M.C., et al. 2018. Alpha-lipoic acid suppresses Nε-(carboxymethyl) lysine-induced epithelial mesenchymal transition in HK-2 human renal proximal tubule cells. Free Radic Res, 1387-1397. |
| 29764240 | Kuo, Y., et al. 2018. Ferrous glycinate regulates cell energy metabolism by restrictinghypoxia-induced factor-1α expression in human A549 cells. Free Radic Res, 1348-1358. |
| 26912141 | Nagai, K., et al. 2016. The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Biomed Res, 63-71. |
| 26902083 | Yao, J., et al. 2016. Assessment of the Cytotoxic and Apoptotic Eἀects of Chaetominine in a Human Leukemia Cell Line. Biomol Ther (Seoul), 147-55. |
| 25740140 | Mohidin, T.B., et al. 2015. BARF1 gene silencing triggers caspase-dependent mitochondrial apoptosis in Epstein-Barr virus-positive malignant cells . J Biosci, 41-51. |
| 25805179 | Perera, Y., et al. 2015. Pharmacologic inhibition of the CK2-mediated phosphorylation of B23/NPM in cancer cells selectively modulates genes related to protein synthesis, energetic metabolism, and ribosomal biogenesis. Mol Cell Biochem, 103-12. |
| 25891083 | Zheng, K.M., et al. 2015. Curcumin inhibits appoptosin-induced apoptosis via upregulating heme oxygenase-1 expression in SH-SY5Y cells. Acta Pharmacol Sin, 544-52. |
| 23098068 | Camgoz, A., et al. 2013. Mechanisms responsible for nilotinib resistance in human chronic myeloid leukemia cells and reversal of resistance . Leuk Lymphoma, 1279-87. |
| 23911596 | Lai, J.H., et al. 2013. Nodal regulates energy metabolism in glioma cells by inducing expression of hypoxia-inducible factor 1α. Neuro Oncol, 1330-41. |
| 23158585 | Selvaraj, V., et al. 2013. Selenium (sodium selenite) causes cytotoxicity and apoptotic mediated cell death in PLHC-1 fish cell line through DNA and mitochondrial membrane potential damage. Ecotoxicol Environ Saf, 80-8. |
| 22079007 | Magalhães, R., et al. 2012. Influence of cell culture configuration on the post-cryopreservation viability of primary rat hepatocytes . Biomaterials, 829-36. |
| 23052231 | Syed, I., et al. 2012. L- threo -C6-pyridinium-ceramide bromide, a novel cationic ceramide, induces NADPH oxidase activation, mitochondrial dysfunction and loss in cell viability in INS 832/13 β-cells. Cell Physiol Biochem, 1051-8. |
| 23023949 | Selvaraj, V., et al. 2012. Protective and antioxidant role of selenium on arsenic trioxide-induced oxidative stress and genotoxicity in the fish hepatoma cell line PLHC-1. Environ Toxicol Chem, 2861-9. |
| 21455605 | Gencer, E., et al. 2011. A novel mechanism of dasatinib-induced apoptosis in chronic myeloid leukemia; ceramide synthase and ceramide clearance genes . Ann Hematol, 1265-75. |
| 21483866 | Rizvi, F., et al. 2011. Mitochondrial dysfunction links ceramide activated HRK expression and cell death. PLoS One, e18137. |
| 21500096 | Kartal, M., et al. 2011. Resveratrol triggers apoptosis through regulating ceramide metabolizing genes in human K562 chronic myeloid leukemia cells . Nutr Cancer, 637-44. |
| 22331716 | Gucluler, G., et al. 2011. The roles of antiapoptotic sphingosine kinase-1 and glucosylceramide genes in drug induced cell death of MCF-7 breast cancer cells . J BUON, 646-51. |
| 21976958 | Liu, C., et al. 2011. SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells. Mol Vis, 2473-81. |
| 20307198 | Baran, Y., et al. 2010. Combination of fludarabine and imatinib induces apoptosis synergistically through loss of mitochondrial membrane potential and increases in caspase-3 enzyme activity in human K562 chronic myleloid leukemia cells. Cancer Invest, 623-8. |
| 20036686 | Sharma, H., et al. 2010. Organochlorine pesticides dieldrin and lindane induce cooperative toxicity in dopaminergic neurons: role of oxidative stress. Neurotoxicology, 215-22. |
| 20815788 | Minamiyama, Y., et al. 2010. Generation of reactive oxygen species in sperms of rats as an earlier marker for evaluating the toxicity of endocrine-disrupting chemicals. Free Radic Res, 1398-406. |
| 20616211 | Yan, H.M., et al. 2010. The oxygen tension modulates acetaminophen-induced mitochondrial oxidant stress and cell injury in cultured hepatocytes. Toxicol Sci, 515-23. |
| 19490758 | Gucluler, G., et al. 2009. Docetaxel enhances the cytotoxic effects of imatinib on Philadelphia positive human chronic myeloid leukemia cells . Hematology, 139-44. |
| 18711728 | Liu, J., et al. 2009. Effects of neuroglobin overexpression on mitochondrial function and oxidative stress following hypoxia/reoxygenation in cultured neurons. J Neurosci Res, 164-70. |
| 19226332 | Lang, M., et al. 2009. Epigallocatechin-gallate modulates chemotherapy-induced apoptosis in human cholangiocarcinoma cells. Liver Int, 670-7. |
| 18022342 | Lengwehasatit, I., et al. 2008. Involvement of B. pseudomallei RpoS in apoptotic cell death in mouse macrophages. Microb Pathog, 238-45. |
| 17936297 | Liu, Z.G., et al. 2007. Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol, 334-45. |
| 16896059 | Bajt, M., et al. 2006. Nuclear translocation of endonuclease G and apoptosis-inducing factor during acetaminophen-induced liver cell injury. Toxicol Sci, 217-25. |
| 16427301 | Panzarini, E., et al. 2006. Apoptosis induction and mitochondria alteration in human HeLa tumour cells by photoproducts of Rose Bengal acetate. J Photochem Photobiol B, 39-47. |
| 15782122 | Liu, Y., et al. 2005. Opposing effects of bovine papillomavirus type 1 E6 and E7 genes on Fas-mediated apoptosis. Oncogene, 3942-53. |
| 15936846 | Ling, X., et al. 2005. Differential expression of survivin-2B and survivin-DeltaEx3 is inversely associated with disease relapse and patient survival in non-small-cell lung cancer (NSCLC). Lung Cancer, 353-61. |
| 15500008 | Yount, G., et al. 2004. Berberine sensitizes human glioma cells, but not normal glial cells, to ionizing radiation in vitro . J Exp Ther Oncol, 137-43. |
| 14970181 | Lawson, B., et al. 2004. Deficiency of the cyclin kinase inhibitor p21(WAF-1/CIP-1) promotes apoptosis of activated/memory T cells and inhibits spontaneous systemic autoimmunity. J Exp Med, 547-57. |