MitoPT JC-1 Assay

from ImmunoChemistry Technologies Product Manual Safety Data Sheet
8 Citations
The MitoPT JC-1 Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, JC-1 reagent is dispersed through the cell and will fluoresce green. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.

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MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay
MitoPT JC-1 Assay

MitoPT JC-1 Assay
SKU: 924

Size: 100 Tests
Price:
$211
Loss of mitochondrial membrane potential is indicative of apoptosis. ICT’s MitoPT JC-1 Assay utilizes the potentiometric fluorescent dye JC-1 to detect mitochondrial membrane permeability and membrane depolarization. The JC-1 dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT JC-1 becomes distributed throughout the cytosol. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
JC-1
Mitochondrial depolarization
488 nm / 590 nm & 527 nm
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
-20°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples.
  2. Create controls with CCCP.
  3. Dilute 10X Assay Buffer 1:10 with diH2O.
  4. Reconstitute MitoPT JC-1 with DMSO.
  5. Dilute MitoPT JC-1 with 1X Assay Buffer.
  6. Add MitoPT JC-1 to each sample.
  7. Incubate 15-30 minutes.
  8. Wash cells: add 1X Assay Buffer and spin cells (twice).
  9. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.
Kit 911: 400 Tests
  • MitoPT JC-1 Reagent, 100 Tests, #6261
  • 10X Assay Buffer, 60 mL, #685
  • CCCP, 50 mM, 125 µL, #6257
  • Kit Manual
    • Kit 924: 100 Tests
  • MitoPT JC-1 Reagent, 100 Tests, #6261
  • 10X Assay Buffer, 60 mL, #685
  • CCCP, 50 mM, 125 µL, #6257
  • Kit Manual

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    Okamoto A, Sumi C, Tanaka H, Kusunoki M, Iwai T, Nishi K, Matsuo Y, Harada H, Takenaga K, Bono H, and Hirota K. HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death. Sci Rep. 2017. Jun 19;7(1):3816. doi: 10.1038/s41598-017-03980-7. Full Text.

    Zou HH, Yang PP, Huang TL, Zheng XX, Xu GS. PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes. Sci Rep. 2017 Jun 27;7(1):4261. doi: 10.1038/s41598-017-00686-8. Full Text.

    Sonntag KC, Ryu WI, Amirault KM, Healy RA, Siegel AJ, McPhie DL, Forester B, Cohen BM. Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles. Sci Rep. 2017. Oct 25;7(1):14038. doi: 10.1038/s41598-017-14420-x. Abstract

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