MitoPT JC-1 Assay
- Prepare samples.
- Create controls with CCCP.
- Dilute 10X Assay Buffer 1:10 with diH2O.
- Reconstitute MitoPT JC-1 with DMSO.
- Dilute MitoPT JC-1 with 1X Assay Buffer.
- Add MitoPT JC-1 to each sample.
- Incubate 15-30 minutes.
- Wash cells: add 1X Assay Buffer and spin cells (twice).
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.
Bülbül, B;Ding, K;Zhan, CG;Çiftçi, G;Yelekçi, K;Gürboğa, M;Özakpınar, ÖB;Aydemir, E;Baybağ, D;Şahin, F;Kulabaş, N;Helvacıoğlu, S;Charehsaz, M;Tatar, E;Özbey, S;Küçükgüzel, İ. Novel 1,2,4-triazoles derived from Ibuprofen: synthesis and in vitro evaluation of their mPGES-1 inhibitory and antiproliferative activity. Molecular diversity. 2022 November 4; doi: 10.1007/s11030-022-10551-0. Full Abstract
Funakoshi, A;Honda, T;Ito, T;Tokura, Y. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations. 2022 September 1; doi: 10.1016/j.xjidi.2022.100153. Abstract
Yoshioka, H;Komura, S;Kuramitsu, N;Goto, A;Hasegawa, T;Amizuka, N;Ishimoto, T;Ozasa, R;Nakano, T;Imai, Y;Akiyama, H. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone and mineral metabolism. 2022 August 10; doi: 10.1007/s00774-022-01354-2. Publication
Zhong, G;Long, H;Zhou, T;Liu, Y;Zhao, J;Han, J;Yang, X;Yu, Y;Chen, F;Shi, S. Blood-brain barrier Permeable nanoparticles for Alzheimer's disease treatment by selective mitophagy of microglia. Biomaterials. 2022 August 8; doi: 10.1016/j.biomaterials.2022.121690. Full Text
Kraemer, BF;Hennis, I;Karge, A;Kraemer, AK;Dreyer, TF;Kiechle, M;Kuschel, B;Bronger, H. Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia. Molecular Medicine (Cambridge, Mass.). 2022 May 4; doi: 10.1186/s10020-022-00472-x. Full Abstract
Chen M, Guan Y, Li A, Zhao YZ, Zhang L, Zhang L, Gong Y. LncRNA SOX2OT Mediates Mitochondrial Dysfunction in Septic Cardiomyopathy. DNA Cell Biol. 2019 Nov;38(11):1197-1206. doi: 10.1089/dna.2019.4839. Epub 2019 Oct 16. Abstract
Ming XY, Fu L, Zhang LY, Qin YR, Cao TT, Chan KW, Ma S, Xie D, Guan XY. Integrin α7 is a functional cancer stem cell surface marker in oesophageal squamous cell carcinoma. Nat Commun. Full text.
Sonntag KC, Ryu WI, Amirault KM, Healy RA, Siegel AJ, McPhie DL, Forester B, Cohen BM. Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles. Sci Rep. 2017. Oct 25;7(1):14038. doi: 10.1038/s41598-017-14420-x. Abstract
Al Wafai R, El-Rabih W, Katerji M, Safi R, El Sabban M, El-Rifai O, Usta J. Chemosensitivity of MCF-7 cells to eugenol: release of cytochrome-c and lactate dehydrogenase. Sci Rep. 2017. 7: 43730. Full text.
Zou HH, Yang PP, Huang TL, Zheng XX, Xu GS. PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes. Sci Rep. 2017 Jun 27;7(1):4261. doi: 10.1038/s41598-017-00686-8. Full Text.
Okamoto A, Sumi C, Tanaka H, Kusunoki M, Iwai T, Nishi K, Matsuo Y, Harada H, Takenaga K, Bono H, and Hirota K. HIF-1-mediated suppression of mitochondria electron transport chain function confers resistance to lidocaine-induced cell death. Sci Rep. 2017. Jun 19;7(1):3816. doi: 10.1038/s41598-017-03980-7. Full Text.
Calvier L, Chouvarine P, Legchenko E, Hoffmann N, Geldner J, Borchert P, Jonigk D, Mozes MM, and Hansmann G. PPARγ Links BMP2 and TGFβ1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism. Cell Metab. 2017. May 2;25(5):1118-1134.e7. doi: 10.1016/j.cmet.2017.03.011. Abstract.
Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N. IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide. J. Immunol. 2017. May 1;198(9):3637-3649. Epub 2017 Mar 31. Abstract.