Nitric Oxide Synthase by Cell Technology

from ImmunoChemistry Technologies Datasheet Safety Data Sheet Protocol

Cell permeable. No wash homogenous assay. Adaptable to High throughput assay platforms . Real time detection of NO activity.

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Nitric Oxide Synthase by Cell Technology
DAF-2DA, the non-fluorescent probe, enters the cells where cytosolic esterases hydrolysis the diacetate groups, releasing the non-fluorescent DAF-2 dye. DAF-2 then reacts with NO and O2 to produce DAF-2T, its triazole fluorescent derivative.
Phase Contrast and fluorescent microscopic images of rat aorta-derived vascular smooth muscular cells loaded with DAF-2DA.
Nitric Oxide Synthase by Cell Technology
Nitric Oxide Synthase by Cell Technology
DAF-2DA, the non-fluorescent probe, enters the cells where cytosolic esterases hydrolysis the diacetate groups, releasing the non-fluorescent DAF-2 dye. DAF-2 then reacts with NO and O2 to produce DAF-2T, its triazole fluorescent derivative.
Phase Contrast and fluorescent microscopic images of rat aorta-derived vascular smooth muscular cells loaded with DAF-2DA.

Nitric Oxide Synthase by Cell Technology
SKU: NOS200-1

Size: 0.125mg
Price:
$205.00

Bulk Order Nitric Oxide Synthase by Cell Technology

Diaminofluorescein –2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable reagent that can measure free Nitric Oxide (NO) and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the diacetate groups on the DFA-2DA reagent arehydrolyzed by cytosolic esterases thus releasing FAD-2 and sequestering the reagent inside the cell. Production of nitric oxide converts the non-fluorescent dye, DAF-2, to its fluorescent triaole derivative, DAF-2T.
Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping, NOT FOR SALE IN JAPAN.
United States
NOS200-1
  • Part# 4006: DAF-2DA, 1 Vial (2-8C)

    • NOS200-2
  • Part# 4006: DAF-2DA, 2 Vials (2-8C)

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