- Prepare samples and controls.
- Dilute 10X Cellular Assay Buffer 1:10 with diH2O.
- Reconstitute FAM-FLICA with 50 µL DMSO.
- Dilute FAM-FLICA 1:5 by adding 200 µL PBS.
- Add diluted FAM-FLICA to each sample at 1:30-1:60 (e.g., spike at 1:30 by adding 10 µL to 290 µL sample).
- Incubate approximately 1 hour.
- Remove media and wash cells 3 times: add 1X Cellular Wash Buffer and spin cells.
- If desired, label with additional stains, such as Hoechst 33342, Propidium Iodide, 7-AAD, or an antibody.
- If desired, fix cells.
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excited at 492 nm and emits at 520 nm.
Kim, D;Ban, K;Lee, G;Jun, H. Lysophosphatidic Acid Induces Podocyte Pyroptosis in Diabetic Nephropathy by an Increase of Egr1 Expression Via Downregulation of EzH2. SSRN Electronic Journal. 2023 January; doi: 10.2139/ssrn.4327442. Abstract
Wang, YH;Zhou, MZ;Ye, T;Wang, PP;Lu, R;Wang, YL;Liu, CX;Xiao, W;Li, JY;Meng, ZB;Xu, LL;Hu, QH;Jiang, C. Discovery of a Series of 5-Amide-1H-pyrazole-3-carboxyl Derivatives as Potent P2Y14R Antagonists with Anti-Inflammatory Characters. Journal of medicinal chemistry. 2022 November 17; doi: 10.1021/acs.jmedchem.2c01632. Article
Yang, Z;Pan, X;Wu, X;Lin, Q;Chen, Y;Cai, S;Zhang, Y;Mai, Z;Ahmad, N;Ma, D;Deng, L. TREM-1 induces pyroptosis in cardiomyocytes by activating NLRP3 inflammasome through the SMC4/NEMO pathway. The FEBS journal. 2022 October 1; doi: 10.1111/febs.16644. Article
Tian, J;Zhou, D;Xiang, L;Xie, B;Wang, B;Li, Y;Liu, X. Calycosin represses AIM2 inflammasome-mediated inflammation and pyroptosis to attenuate monosodium urate-induced gouty arthritis through NF-κB and p62-Keap1 pathways. Drug Development Research. 2022 September 7; doi: 10.1002/ddr.21985. Text
Martino, E;Balestrieri, A;Anastasio, C;Maione, M;Mele, L;Cautela, D;Campanile, G;Balestrieri, ML;D'Onofrio, N. SIRT3 Modulates Endothelial Mitochondrial Redox State during Insulin Resistance. Antioxidants (Basel, Switzerland). 2022 August 19; doi: 10.3390/antiox11081611. Abstract
Su, M;Chen, C;Li, S;Li, M;Zeng, Z;Zhang, Y;Xia, L;Li, X;Zheng, D;Lin, Q;Fan, X;Wen, Y;Liu, Y;Chen, F;Luo, W;Bu, Y;Qin, J;Guo, M;Qiu, M;Sun, L;Liu, R;Wang, P;Hwa, J;Tang, WH. Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis. Nature cardiovascular research. 2022 August 4; doi: 10.1038/s44161-022-00108-7. Citation
He, S;Li, L;Chen, H;Hu, X;Wang, W;Zhang, H;Wei, R;Zhang, X;Chen, Y;Liu, X. PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome Activation. Journal of Virology. 2022 July 27; doi: 10.1128/jvi.02127-21. Article
Xiong, J; Ran, L; Zhu, Y; Wang, Y; Wang, S; Wang, Y; Lan, Q; Han, W; Liu, Y; Huang, Y; He, T; Li, Y; Liu, L; Zhao, J; Yang, K. DUSP2-mediated inhibition of tubular epithelial cell pyroptosis confers nephroprotection in acute kidney injury. Theranostics. 2022 July 4; doi: 10.7150/thno.72291. Article
Hung, SC;Ke, LC;Lien, TS;Huang, HS;Sun, DS;Cheng, CL;Chang, HH. Nanodiamond-Induced Thrombocytopenia in Mice Involve P-Selectin-Dependent Nlrp3 Inflammasome-Mediated Platelet Aggregation, Pyroptosis and Apoptosis. Frontiers in Immunology. 2022 April 4; doi: 10.3389/fimmu.2022.806686. Full Text
Liu, M;Luo, G;Liu, T;Yang, T;Wang, R;Ren, W;Liu, P;Lai, X;Zhou, H;Yang, S. Zhilong Huoxue Tongyu Capsule Alleviated the Pyroptosis of Vascular Endothelial Cells Induced by ox-LDL through miR-30b-5p/NLRP3. Evidence-based complementary and alternative medicine : eCAM. 2022 January 27; doi: 10.1155/2022/3981350. Full Article
Chen, A;Chen, Z;Zhou, Y;Wu, Y;Xia, Y;Lu, D;Fan, M;Li, S;Chen, J;Sun, A;Zou, Y;Qian, J;Ge, J. Rosuvastatin protects against coronary microembolization-induced cardiac injury via inhibiting NLRP3 inflammasome activation. Cell Death & Disease. 2021 Jan 12; 12(1)78. doi: 10.1038/s41419-021-03389-1. Full Article