Detect active caspase enzymes with the SR FLICA® Poly Caspase Assay Kit. This in vitro assay employs the fluorescent inhibitor probe SR-VAD-FMK to label active caspase enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

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SR-FLICA® Poly Caspase Assay Kit
SKU: 916

Size: 25 Tests
Price:
Sale price$201


Bulk Order SR-FLICA® Poly Caspase Assay Kit

Caspases play important roles in apoptosis and inflammation. ICT’s FLICA poly caspase assay kit is used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.
SR-VAD-FMK
Poly caspases
550-580 nm / 590-600 nm
Flow Cytometer, Fluorescence Plate Reader, Fluorescence Microscope
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples and controls.
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
  3. Reconstitute FLICA with 50 μL DMSO.
  4. Dilute FLICA 1:5 by adding 200 μL PBS.
  5. Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells.
  6. Incubate approximately 1 hour.
  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells.
  8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody.
  9. If desired, fix or embed cells.
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.
Kit 916: 25 Tests
  • FLICA Poly Caspase Reagent (SR-VAD-FMK), 1 vial, #679
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Kit 917: 100 Tests
  • FLICA Poly Caspase Reagent (SR-VAD-FMK), 4 vials, #679
  • 10X Apoptosis Wash Buffer, 60 mL, #634
  • Fixative, 6 mL, #636
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Wu, R;Ohara, RA;Jo, S;Liu, TT;Ferris, ST;Ou, F;Kim, S;Theisen, DJ;Anderson, DA;Wong, BW;Gershon, T;Schreiber, RD;Murphy, TL;Murphy, KM. Mechanisms of CD40-dependent cDC1 licensing beyond costimulation. Nature Immunology. 2022 October 21; doi: 10.1038/s41590-022-01324-w. Full Abstract

    Nougarede A, Popgeorgiev N, Kassem L, Omarjee S, Borel S, Mikaelian I, Lopez J, Gadet R, Marcillat O, Treilleux I, Villoutreix BO, Rimokh R, Gillet G. Breast cancer targeting through inhibition of the endoplasmic reticulum-based apoptosis regulator Nrh/BCL2L10. Cancer Res. 2018. Jan 12. pii: canres.0846.2017. doi: 10.1158/0008-5472.CAN-17-0846. [Epub ahead of print]. Abstract

    Schell SL, Soni C, Fasnacht MJ, Domeier PP, Cooper TK, Rahman ZSM. Mer Receptor Tyrosine Kinase Signaling Prevents Self-Ligand Sensing and Aberrant Selection in Germinal Centers. J. Immunol. Dec 15;199(12):4001-4015. doi: 10.4049/jimmunol.1700611. Epub 2017 Nov 8. Abstract

    Marschall M, Simnacher U, Walther P, Essig A, Hagemann J. The Putative Type III Secreted Chlamydia abortus Virulence-Associated Protein CAB063 Targets Lamin and Induces Apoptosis. Front Microbiol. 2020 May 25;11:1059. doi: 10.3389/fmicb.2020.01059. eCollection 2020. Full Text

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