Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein. Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal. Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency. Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.
Cell Technology's Synthetic Blocker is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because Synthetic Blocker contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
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7.4 at 1X
All synthetic materials
Expires two years from date of manufacture
Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).