TMB 1-Component HRP Membrane Substrate (SUBM)
TMB 1-Component HRP Membrane Substrate is a ready-to-use formulation containing 3,3′,5,5′-tetramethylbenzidine in a mildly acidic buffer. This TMB substrate is useful for immunoblotting applications where HRP-conjugated molecules are used for detection. TMB 1-Component HRP Membrane Substrate reacts with HRP yielding a dark blue reaction product.
TMB 1-Component HRP Membrane Substrate (SUBM)
TMB 1-Component HRP Membrane Substrate is a ready-to-use formulation containing 3,3′,5,5′-tetramethylbenzidine in a mildly acidic buffer. SUBM is useful for immunoblotting applications where HRP-conjugated molecules are used for detection. SUBM reacts with HRP yielding a dark blue reaction product.
Bulk volumes are available upon request.
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
5.25-5.65
1X
United States
3 years from date of manufacture
- Allow product to equilibrate to room temperature (25°C) prior to use.
- Completely cover the membrane surface with the substrate. The substrate will react with peroxidase creating an insoluble permanent dark blue reaction product.
- To stop the reaction, rinse the developed membrane with high quality water.
- For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.