Apoptosis and Autophagy Kits

66 products

Apoptosis is a process of programmed cell death that occurs in multicellular organisms. It is a highly regulated and controlled process that occurs normally during development and aging as a homeostatic mechanism to maintain cell populations.

Autophagy plays a critical role in maintaining homeostasis by preventing the accumulation of damaged organelles by disassembling unnecessary or dysfunctional cells and cellular components. Autophagy occurs at low levels in the cell under normal conditions and can be rapidly upregulated during times of starvation or stress.

ICT offers a full line of products to induce apoptosis, including FLICA, FLIVO, Annexin kits and more; our Autophagy Assay - Red Fluorescent enables researchers to detect and monitor the in vitro development of autophagy in living cells.

Showing 1 - 24 of 66 products

Figure 1. Flow cytometry analysis of active caspase-9 in MDA-MB-231 (A,B) and ZR-75-1 (C,D) cells. Cells were stained with FAM-FLICA® Caspase-9 reagent. From Figure 5 of R. Krętowski and M. Cechowska-Pasko, The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells, Int J Mol Sci (2022) 23, 9285. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 by the authors.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

ImmunoChemistry Technologies Tumor Necrosis Factor beta

$360

ImmunoChemistry Technologies Tumor Necrosis Factor alpha

$664

Figure 1. Poly Caspase SR-VAD-FMK Kit (SR100-2) and Poly Caspase FAM-VAD-FMK (FAM100-2) determine apoptosis in B cells after EBV infection. Figure 4C of D. Westhoff Smith et al, The Epstein-Barr Virus Oncogene EBNA1 Suppresses Natural Killer Cell Responses and Apoptosis Early after Infection of Peripheral B Cells (2021), mBio 12 (6) e02243-21 doi: 10.1128/mBio.02243-21. Used under the Creative Commons Attribution (https://creativecommons.org/licenses/by/4.0/). Copyright © 2021 by the Authors.

ImmunoChemistry Technologies Poly Caspase SR-VAD-FMK Kit

From $212

Figure 1. Caspase activation in DAL-1/4.1B-induced MCF-7 cells. Caspase 10 was measured by FACS using FAM-AEVD-FMK. From Figure 2B of W. Jiang & I. F. Newsham, The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells, Mol Cancer (2006) 5, 4 doi: 10.1186/1476-4598-5-4. Used under Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0). Copyright © 2006 Jiang & Newsham.

ImmunoChemistry Technologies FAM-AEVD- FMK Caspase 10 Detection Kit

From $212

Figure 1. Caspase activation in DAL-1/4.1B-induced MCF-7 cells. Caspase 2 was measured by FACS using FAM-VDVAD-FMK. From Figure 2B of W. Jiang & I. F. Newsham, The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells, Mol Cancer (2006) 5, 4 doi: 10.1186/1476-4598-5-4. Used under Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0). Copyright © 2006 Jiang & Newsham.

ImmunoChemistry Technologies FAM-VDVAD-FMK Caspase 2 Detection Kit

From $233

Figure 1. Caspase activation in DAL-1/4.1B-induced MCF-7 cells. Caspase 1 was measured by FACS using FAM-YVAD-FMK. From Figure 2B of W. Jiang & I. F. Newsham, The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells, Mol Cancer (2006) 5, 4 doi: 10.1186/1476-4598-5-4. Used under Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0). Copyright © 2006 Jiang & Newsham.

ImmunoChemistry Technologies FAM-YVAD-FMK Caspase 1 Detection Kit

From $222

Figure 1. Caspase activation in DAL-1/4.1B-induced MCF-7 cells. Caspase 6 was measured by FACS using FAM-VEID-FMK. From Figure 2B of W. Jiang & I. F. Newsham, The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells, Mol Cancer (2006) 5, 4 doi: 10.1186/1476-4598-5-4. Used under Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0). Copyright © 2006 Jiang & Newsham.

ImmunoChemistry Technologies FAM-VEID-FMK Caspase 6 Detection Kit

From $233

Figure 1. Effect of various treatments on caspase 9 activity in rat cardiomyocytes. Cells were labeled with FAM-LEHD-FMK and analyzed by flow cytometry. From Figure 3C of X. Lv et al, Berberine Inhibits Doxorubicin-Triggered Cardiomyocyte Apoptosis via Attenuating Mitochondrial Dysfunction and Increasing Bcl-2 Expression, PLoS One (2012) 7, e47351 doi: 10.1371/journal.pone.0047351. Used under Creative Commons Attribution License. Copyright © 2012, Lv et al.

ImmunoChemistry Technologies FAM-LEHD-FMK Caspase 9 Detection Kit

From $222

Figure 1. Effect of various treatments on caspase 8 activity in rat cardiomyocytes. Cells were labeled with FAM-LETD-FMK and analyzed by flow cytometry. From Figure 3B of X. Lv et al, Berberine Inhibits Doxorubicin-Triggered Cardiomyocyte Apoptosis via Attenuating Mitochondrial Dysfunction and Increasing Bcl-2 Expression, PLoS One (2012) 7, e47351doi: 10.1371/journal.pone.0047351. Used under Creative Commons Attribution License. Copyright © 2012, Lv et al.

ImmunoChemistry Technologies FAM-LETD-FMK Caspase 8 Detection Kit

From $222

Fig A. Jurkat cells treated with DMSO. Cells were labeled with FAM-VAD-FMK for 1 hour. Caspase activity was detected using flow cytometry.

ImmunoChemistry Technologies Casp 3/7 FAM-DEVD-FMK by Cell Technology

From $212

ImmunoChemistry Technologies Poly Caspase FAM-VAD-FMK

From $212

Figure 1. Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

Figure 2. Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti-active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active PE - Caspase 3 Detection Kit

$506

Figure 1. Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with anti-active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti-Rabbit antibody.

ImmunoChemistry Technologies Apo Active FITC - Caspase 3 Detection Kit

$506

Figure 1. U937 cells were stimulated with staurosporine for 5 hours, and then assayed for caspase 3/7 activity following the kit protocol.

ImmunoChemistry Technologies Apo 3/7 HTS Assay Kit

From $310

Figure 1. Balb/c mice were either inoculated with HSV-1 virus, or given a sham treatment. Seven days later, the mice were injected IV with either DyLight® 747-VAD-FMK (kit 9114), NIR-DyLight® 747 Free Dye (kit 9115), or no reagent. Strong caspase activity was seen in the brain of the HSV-1 infected / NIR-DyLight® 747 Tracer-treated mouse. The liver is the route of clearance for DyLight® Tracers. All mice show fluorescence signal in the tail where reagent is likely to pool after IV injection.

ImmunoChemistry Technologies Near Infrared DyLight® 747 Free Dye Control Assay

$302

Figure 1. Apoptotic tissues in murine tumor models were imaged in vivo with 747-VAD-FMK (cat. 9114). Negative control (placebo) mice (left) and experimentally treated mice (right) were injected intravenously with DyLight® 747-VAD-FMK tracer and imaged non-invasively at various time points with the CRi Maestro™ imaging system. Apoptotic tumor tissues fluoresce bright blue in the experimental group subjects, indicating that the experimental treatment had an apoptotic effect.

ImmunoChemistry Technologies Near-Infrared DyLight® 747 Tracer in vivo Active Caspase (VAD) Assay

$438

Figure 1. Brain abscesses were induced in mice following inoculation of live S. aureus. Mice received i.v. injections of DyLight® 690-VAD-FMK tracer or DyLight® 690 Dye Control (cat. 9113) 17 hours post-infection; brain tissue was imaged 1 hour later ex vivo using an IVIS® Spectrum™ (Caliper Life Sciences). Strong caspase activity was seen in brain abscesses using DyLight® 690-VAD-FMK tracer (right); minimal signal was detected in the DyLight® 690 Free Dye Control (left).

ImmunoChemistry Technologies Near Infrared DyLight® 690 Free Dye Control Assay

$302

ImmunoChemistry Technologies Near-Infrared DyLight® 690 Tracer in vivo Active Caspase (VAD) Assay

$438

Figure 1. Excitation and emission spectra. Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 630 nm. The emission spectrum (orange) was generated using an excitation of 540 nm. The probe optimally excites at 550-580 nm and has a peak emission at 590-600 nm.

Figure 2. Mice were given either a 0Gy or 15Gy whole lung dose of gamma irradiation. SR in vivo probe (Cat. 982/983) was injected and allowed to circulate for 18 hours prior to sacrifice. Paraffin sections were prepared, nuclei stained with DAPI (blue), and lungs imaged with a fluorescence microscope. Caspase activity (red) was induced in irradiated mice compared to control animals. Data courtesy of E. Hernady (University of Rochester Medical Center, Rochester, NY).

ImmunoChemistry Technologies Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay

From $205.50

Figure 1. Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay inhibitor probe was reconstituted in DMSO, diluted in an aqueous buffer, and then analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.

Figure 2. Staurosporine (cat. 6212) was injected into the forebrain of a female house sparrow to induce apoptosis. Approximately 20 hours later, FAM in vivo probe (cat. 980/981) was intravenously injected into the jugular and allowed to circulate 30 minutes. The bird was sacrificed and frozen brain sections prepared. Neurons with active caspases fluoresce green. This image shows one apoptotic neuron at 100X. Data courtesy of Mr. Chris Thompson at the University of Washington, Seattle.

ImmunoChemistry Technologies Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay

From $205

Figure 1. Using FAM-FLICA®, non-apoptotic cells (unstained, left of histogram) can easily be distinguished from apoptotic cells exhibiting green fluorescence (right of histogram). Jurkat cells were either treated with DMSO as control (A), or 1 µM staurosporine (B) to induce apoptosis. Cells were stained with FAM-FLICA® and analyzed using a flow cytometer in FL-1. Only 7% of non-induced cells (A, right) are apoptotic compared with 96.5% of the induced cells (B, right). (ICT 226:17-19.)

Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were either untreated (A), or treated with 90% ethanol for 60 seconds, then stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.

ImmunoChemistry Technologies Necrosis vs Apoptosis Assay Kit

From $340

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained using Swine Annexin V-Fluorescein Assay (cat. 9142). Annexin V-Fluorescein labeled only 13.1% of control cells (UR + LR = 11.0% + 3.1%, left), whereas staurosporine induced Annexin V-Fluorescein labeling in 96.7% of cells (UR + LR = 32.7% + 64.0%, right). Staurosporine also significantly increased the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:19.

ImmunoChemistry Technologies Swine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Equine Annexin V-Fluorescein Assay (Cat. 9141). Equine Annexin V-Fluorescene labeled only 17.4% of the control cells (UR + LR = 10.3% + 7.1%, left); staurosporine induced Equine Annexin V-Fluorescene labeling in 97.6% of cells (UR + LR = 27.8% + 69.8%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:68.

ImmunoChemistry Technologies Equine Annexin V-Fluorescein Apoptosis Assay Kit

$455

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Apoptosis and Autophagy Kits

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