ICT’s Pyroptosis/Caspase-1 Assay Kits utilize our popular FLICA® technology to detect caspase-1 activation. These kits contains the caspase-1 inhibitor reagents, which have the preferred binding sequence for caspase-1, Tyr-Val-Ala-Asp (YVAD). These kits also contain Nigericin, a potent microbial toxin derived from Streptomyces hygroscopicus, that acts as a potassium ionophore, capable of inducing a net decrease in intracellular levels of potassium which is crucial for oligomerization of the NLRP3 inflammasome and activation of caspase-1.