Oxidative Stress

15 products

Oxidative stress reflects an imbalance between the production of reactive oxygen and nitrogen species and the body's ability to detoxify reactive intermediates. Nitric oxide synthases are a family of enzymes capable of catalyzing the production of nitric oxide (NO). NO is an important molecule that is involved in regulating a variety of cellular processes such as angiogenesis, peristalsis, and the immune response to invading pathogens. Reactive nitrogen species (RNS) are a family of antimicrobial molecules derived from NO and superoxide through the enzymatic activities of nitric oxide synthase (NOS).

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cellular structures.

ICT carries products for detecting oxidative or nitrosative stress.

Showing 1 - 15 of 15 products

ImmunoChemistry Technologies Chlorination and Oxidation Detection Kit by Cell Technology

$682

Figure 1. Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10 mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL).

Figure 2. Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488 nm emission: 505-550 nm barrier filters. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.

ImmunoChemistry Technologies Fluorescent Hypochlorite Detection Kit

$506

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin.

Figure 2. Typical Hydrogen peroxide standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Hydrogen Peroxide Detection/Peroxidase Detection Kit

$279

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained using Swine Annexin V-Fluorescein Assay (cat. 9142). Annexin V-Fluorescein labeled only 13.1% of control cells (UR + LR = 11.0% + 3.1%, left), whereas staurosporine induced Annexin V-Fluorescein labeling in 96.7% of cells (UR + LR = 32.7% + 64.0%, right). Staurosporine also significantly increased the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:19.

ImmunoChemistry Technologies Swine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.

Figure 2. Jurkat suspension cells were stained with DAF-2DA dye, washed, and then treated with DMSO control (Panels A-C) or DEA NONOate (Panels D-F), a nitric oxide donor. Cells treated with DEA NONOate showed an increased level of green fluorescence relative to the untreated cells (Panels A v and D). Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 228:57-58).

ImmunoChemistry Technologies Nitric Oxide Synthase Assay

$216

Figure 1. Jurkat cells were stained with the Intracellular Total ROS Green dye (cat. 9144), then treated with a negative control (left) or 100 µM tert-Butyl hydroperoxide (ROS-inducing agent) (middle). The median fluorescence intensity (MFI) of stained negative control cells was 11,875 in FL1-A (Negative) and 419,067 for ROS-induced cells (Positive). The data are also shown in overlay (black: Negative, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.

ImmunoChemistry Technologies Intracellular Total ROS Activity Assay

$341

Figure 2. Recovery of 8-OHdG from urine. Urine samples were spiked with 8-OHdG, diluted as described in the protocol, analyzed using the 8-OHdG ELISA Kit. The y-intercept corresponds to the amount of 8-OHdG in unspiked urine. Error bars represent standard deviations obtained from multiple dilutions of each sample.

ImmunoChemistry Technologies DNA Damage ELISA Kit

$657

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (middle), then stained using Intracellular GSH Assay (cat. 9137). Cells were read on a flow cytometer using an FL1 99% (2 log) attenuation filter. The median fluorescence intensity of negative control cells was 425,971 in FL1-A (left: Negative), and 289,169 in the induced cells (middle: Positive), a decrease of >30%. Overlay: green: Negative, red: Positive. Data courtesy of Ms. T. Hanson, ICT, 216:52, 051612.

ImmunoChemistry Technologies Intracellular GSH Assay Kit

$238

Figure 1. Typical standard curves. A new curve must be generated each time the assay is run.

Figure 2. Typical nitrite linearity curve

ImmunoChemistry Technologies Nitric Oxide Colorimetric Detection Kit

$339.50

ImmunoChemistry Technologies Glutathione Colorimetric Detection Kit

$442

ImmunoChemistry Technologies Glutathione Fluorescent Detection Kit

From $427.50

Figure 1. Typical standard curve. A new curve must be generated each time the assay is run.

ImmunoChemistry Technologies Hydrogen Peroxide Colorimetric Detection Kit

$441

ImmunoChemistry Technologies Hydrogen Peroxide Fluorescent Detection Kit

$441

Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$290

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine to induce apoptosis, then stained with TMRE, and photographed using a photomicroscope using a green excitation filter at 510-560 nm and a 570-620 nm emission filter (left). Healthy cells, containing mitochondria with polarized inner membranes fluoresce bright orange (A) compared with apoptotic cells with depolarized mitochondria(B). The corresponding DIC images are shown (right) (Dr. Brian Lee, ICT).

Figure 2. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRE - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$290

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Oxidative Stress

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