Experienced ELISA development, tailored to achieve your goals.
ELISA (Enzyme-Linked ImmunoSorbent Assay) is a widely-used assay for quantification of analytes in a sample, and has long been used as the gold-standard in both clinical and research laboratories. Projects that we run for our clients vary from routine outsourced ELISA assays, to more sophisticated assay development for clinical samples, contaminant testing, and other applications.
Assay Development Process Overview
Design Input
- We discuss the purpose of your assay and help you decide on format and configuration.
Antibody Selection
- Certain antibody combinations are best for specific assay types. For example, in sandwich ELISAs, one antibody traps the analyte, and the other antibody is used to detect. These pairs are tested for compatibility.
Formatting
- We ensure the antibodies are properly attached to solid phase for optimal reactivity.
Prototype Development, Pilot Studies
- We test standard samples, matrices, conjugates, etc. in combination to create the prototype.
- We help you select standards and set aside key controls, etc.
Technical Transfer
- We transfer the technology to your laboratory, ensuring that the assay works in your technicians' hands.
ELISA Development
Looking to detect or measure a specific target molecule? We can fully develop a quick, accurate, and reliable immunoassay test or optimize an existing assay.
Conjugation
Covalently attach signal-generating enzymes to antibodies, couple fluorescent labels to specific proteins and peptides, biotinylate antibodies and proteins, or conjugate hapten groups. We do it all, and do it quickly and efficiently.
Lyophilization
Need something vialed and freeze-dried? We can prepare it for you with our custom lyophilization services.
Plate Coating
If you need coated microtiter plates quickly, contact us. We will coat, block, dry, and seal your microtiter plates in foil bags with a desiccant packet so they are ready to use the next time you need to run a sample.
Purification
We can assist you with your antibody purification requirements, purifying and concentrating monoclonal and polyclonal antibodies using Protein G or Protein A. We can also affinity purify polyclonal IgG samples.
Consultation
With our extensive experience in immunochemistry, we have encountered and solved just about every issue. Call us for help in building an assay from start to finish, or optimizing an existing assay that is not performing as well as you need. High backgrounds or low specific signal? We have the solution.
Ready to Get Started?
For questions or a quote from one of our scientists, submit your project details below. We will respond within two business days. If you require immediate assistance, call us at (800) 824-8540.