ImmunoChemistry Technologies’ serine protease inhibitor assays enable researchers to detect intracellular chymotrypsin-like serine protease activity in vitro without lysing the cell. The reagents are non-cytotoxic green or red fluorescent inhibitors that covalently bind with active serine protease enzymes.
It is a powerful method to assess the intracellular levels of chymotrypsin-like serine protease activity in vitro. Just add the serine protease inhibitor probe directly to the cell culture media, incubate, and wash. Because these inhibitors are cell-permeant, they will efficiently diffuse in and out of all living cells. If there is an active chymotrypsin-like enzyme inside the cell, it will covalently bind with the serine protease inhibitor and retain the green or red fluorescent signal within the cell.
