This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or our Universal Immuno buffer.
- The tissue slides are immersed in this 1X solution and heated for 10-20 minutes at 100ºC; OR in pressure cooker for 3-5 minutes followed by cooling at room temperature for about 20 minutes.
- The tissue sections are ready for further IHC protocol.