Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. Cell permeablity permits direct visualization of cytosolic apoptotic events. Apoptotic cell population does not diminish over time. Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure. Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments. Yields both quantitative and qualitative results. Gives strong signal with little background noise.
FAM700-2: 100 Tests