Hydrogen Peroxide Detection/Peroxidase Detection Kit

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A rapid, no-wash assay to measure H2O2, based on the formation of a fluorescent product. The kit can measure H2O2 release from cells or enzyme coupled reactions. It can also measure peroxidase activity.

Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin.
Figure 2. Typical Hydrogen peroxide standard curve. A new curve must be generated each time the assay is run.
Figure 3. Peroxidase titration.
Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin.
Figure 1. Assay principle. The kit uses a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin.
Figure 2. Typical Hydrogen peroxide standard curve. A new curve must be generated each time the assay is run.
Figure 3. Peroxidase titration.

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Data Sheet Safety Data Sheet

SKU: FLOH100-3

Size: 500 Tests
Price:
$279.00
The kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions.
Fluorescence plate reader
2-8°C
Ships overnight (domestic), International Priority Shipping
United States
  • Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C)
  • Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C)
  • Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C)
  • Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)

    • Product Specific References

    PMID Publication
    33918273Ola, M.S., et al. 2021. Does Hyperglycemia Cause Oxidative Stress in the Diabetic Rat Retina?. Cells, 794.
    31664127Zhu, Y, et al. 2019. Novel dihydroartemisinin dimer containing nitrogen atoms inhibits growth of endometrial cancer cells and may correlate with increasing intracellular peroxynitrite. Scientific Reports, 15528.
    29906250Tripathi, J.K., et al. 2018. Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection. The FASEB Journal, fj201800605.

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