Quick 10 minute assay. Can monitor multiple time points to follow kinetics. Dual mode, can detect H202 or peroxidase activity. One-step, no wash assay. Adaptable for High Throughput format. Non-destructive cell based assay allows monitoring of additional parameters.

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Fluoro H2O2 by Cell Technology
SKU: FLOH100-3

Size: 500 Tests
Sale price$270.00

Bulk Order Fluoro H2O2 by Cell Technology

The Fluoro H2O2 detection kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions.
Fluorescence plate reader
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United States
  • Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C)
  • Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C)
  • Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C)
  • Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)
  • Product Specific References

    PMID Publication
    33918273Ola, M.S., et al. 2021. Does Hyperglycemia Cause Oxidative Stress in the Diabetic Rat Retina?. Cells, 794.
    31664127Zhu, Y, et al. 2019. Novel dihydroartemisinin dimer containing nitrogen atoms inhibits growth of endometrial cancer cells and may correlate with increasing intracellular peroxynitrite. Scientific Reports, 15528.
    29906250Tripathi, J.K., et al. 2018. Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection. The FASEB Journal, fj201800605.

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