Fluoroshield™

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.



SKU: AR-6500-01

Volume: 20 ml
Price:
Sale price$85.75
Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cyanine 2, Cyanine 3, Cyanine 5, Alexa Fluor 488, Alexa Fluor 594, green fluorescent protein (GFP), tetramethyly rhodamine, Redox, phycoerythrin (R-PE), phyocyanin (PC), and allophycocyanin (APC). The fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of cyanine dyes, R-PE, PC and APC.
Ready to use mounting medium
Immunofluorescence, confocal microscopy
Room temp; Long term storage 2-8°C recommended; Protect from light; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
  1. Bring mounting medium vial to room temperature.
  2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
  3. Turn the vial upside down and open the dropper to remove any air bubbles.
  4. Apply 1-3 drops of mounting medium directly on top of the specimen.
  5. Let stand at room temperature for about 3-5 minutes in the dark.
  6. Apply cover slip carefully avoiding air bubbles.
  7. The specimen is ready for visualization under a microscope.
  8. One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (cat. # AR-6504). If a coverslip is not sealed air bubbles will appear in few days.
  9. Method for applying Coverslip: Put 1-3 drops of FLS on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37C for one hour in the dark to dry organic mounting medium.
  10. For storage it is recommended that the slide be stored in the dark at 2-8oC.

Product Specific References

PMID Publication
37555698Yasuda, T, et al. 2023. Retinal regeneration after injury induced by gamma-ray irradiation during early embryogenesis in medaka, Oryzias latipes. International Journal of Radiation Biology, .
37246520Ono, Y, et al. 2023. Scleraxis-lineage cells are required for correct muscle patterning. Development, dev201101.
37106052Baek, MJ, et al. 2023. Tailoring renal-clearable zwitterionic cyclodextrin for colorectal cancer-selective drug delivery. Nature Nanotechnology, 945-956.
36900417Nadir, A, et al. 2023. Pro-Apoptotic and Anti-Cancer Activity of the Vernonanthura Nudiflora Hydroethanolic Extract. Cancers, 1627.
36578022Verma, A, et al. 2022. Targeting the overexpressed mitochondrial protein VDAC1 in a mouse model of Alzheimer's disease protects against mitochondrial dysfunction and mitigates …. Translational Neurodegeneration, 58.
36291596Anand, U, et al. 2022. The multicellular effects of VDAC1 N-terminal-derived peptide. Biomolecules, 1387.
36207753Suzuka, T., et al. 2022. Therapeutic effects of adipose-derived mesenchymal stem/stromal cells with enhanced migration ability and hepatocyte growth factor secretion by low …. Arthiritis Research and Therapy, 228.
36077343Shteinfer-Kuzmine, A., et al. 2022. Targeting the mitochondrial protein VDAC1 as a potential therapeutic strategy in ALS. International Journal of Molecular Science, 9946.
35831437Taketomi, T, et al. 2022. Autism-associated mutation in Hevin/Sparcl1 induces endoplasmic reticulum stress through structural instability. Scientific reports, 11891.
34750505Kakihara, K, et al. 2021. Molecular basis of ubiquitin-specific protease 8 autoinhibition by the WW-like domain. Communications Biology, 1272.
34526560Yano, S, et al. 2021. A MATLAB-based program for three-dimensional quantitative analysis of micronuclei reveals that neuroinflammation induces micronuclei formation in the …. Scientific reports, 18360.
34189280Fukunaga, K, et al. 2021. Protocadherin-1 is expressed in the notochord of mouse embryo but is dispensable for its formation. Biochemisty and Biophysics Reports, 101047.
33671550Al-Maawi, S, et al. 2021. Biologization of Pcl-Mesh Using Platelet Rich Fibrin (Prf) Enhances Its Regenerative Potential In Vitro. International journal of molecular sciences, 2159.
33319419Ikuta, R, et al. 2021. N‐cadherin localization in taste buds of mouse circumvallate papillae. The Journal of Comparative Neurology, 2227-2242.
33339841Taniguchi, T., et al. 2020. Novel use of a chemically modified siRNA for robust and sustainable in vivo gene silencing in the retina. Scientific Reports, 22343.
32839239Kim, J, et al. 2020. USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis. Molecular and cellular biology, e00098-20.
32829670Dohle, E, et al. 2020. Co-culture Model for Cutaneous Wound Healing to Assess a Porous Fiber-Based Drug Delivery System. Tissue Engineering. Part C, Methods, 475-484.
32378355Stangelin, E, et al. 2020. Bone Scaffolds Based on Degradable Vaterite/PEG-Composite Microgels. Advanced Healthcare Materials, e1901820.
32238697Hirao-Suzuki, M, et al. 2020. Cannabidiolic acid dampens the expression of cyclooxygenase-2 in MDA-MB-231 breast cancer cells: Possible implication of the peroxisome proliferator-activated receptor β/δ abrogation. The Journal of Toxilogical Sciences, 227-236.
31157513Yokoyama, R, et al. 2019. Cardiac Regeneration by statin-polymer nanoparticle-loaded adipose-derived stem cell therapy in myocardial infarction. Stem Cells Translational Medicine, 1055-1067.
30963526Herrera-Vizcaíno, C, et al. 2019. Platelet-rich fibrin secretome induces three dimensional angiogenic activation in vitro. European Cells and Materials, 250-264.
30101647Dohle, E, et al. 2018. Human Co- and Triple-Culture Model of the Alveolar-Capillary Barrier on a Basement Membrane Mimic. Tissue Engineering. Part C, Methods, 495-503.
29770592Schröder, R., et al. 2018. Particles of vaterite, a metastable CaCO3 polymorph, exhibit high biocompatibility for human osteoblasts and endothelial cells and may serve as a biomaterial for …. Journal of Tissue Engineerying and Regenerative Medicine, 1754-1768.
29724225Frith, KA, et al. 2018. Towards development of aptamers that specifically bind to lactate dehydrogenase of Plasmodium falciparum through epitopic targeting. Malaria Journal, 191.
28715156Herath, T.D., et al. 2018. Neutrophil‐mediated enhancement of angiogenesis and osteogenesis in a novel triple cell co‐culture model with endothelial cells and osteoblasts. Journal of Tissue Engineering and Regenerative Medicine, e1221-e1236.
28509340Dohle, E, et al. 2018. Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering. Journal of tissue engineering and regenerative medicine, 598-610.
29262348Sander, J, et al. 2017. Cellular differentiation of human monocytes is regulated by time-dependent interleukin-4 signaling and the transcriptional regulator NCOR2. Immunity, 1051-1066.
29097816Kotani, T, et al. 2017. Anti-inflammatory and anti-fibrotic effects of intravenous adipose-derived stem cell transplantation in a mouse model of bleomycin-induced interstitial pneumonia. Scientific Reports, 14608.
28699196Nishimura, S, et al. 2017. Cytokeratin-positive folliculo-stellate cells in chicken adenohypophysis. Animal Science Journal, 1835-1841.
28597061Riew, TR, et al. 2017. Progressive accumulation of autofluorescent granules in macrophages in rat striatum after systemic 3-nitropropionic acid: a correlative light-and electron-microscopic …. Histochemistry and Cell Biology, 517-528.
28574635Nishimura, S, et al. 2017. Estradiol inhibits hepatic stellate cell area and collagen synthesis in the chicken liver. Animal Science Journal, 1827-1834.
28127104Kawabe, Y., et al. 2016. Morphological changes of myoepithelial cells in the rat submandibular gland following the application of surgical stimuli. Acta Histochemica et Cytochemica, 159-169.
26773979Bhunia, SK, et al. 2016. Imaging cancer cells expressing the folate receptor with carbon dots produced from folic acid. Chembiochem, 614-619.
26683873Nagata, H, et al. 2016. Cardiac adipose-derived stem cells exhibit high differentiation potential to cardiovascular cells in C57BL/6 mice. Stem Cells Translational Medicine, 141-151.
26077869Ohta, M, et al. 2015. Two chalcones, 4-hydroxyderricin and xanthoangelol, stimulate GLUT4-dependent glucose uptake through the LKB1/AMP-activated protein kinase signaling pathway …. Nutrition Research, 618-625.
24992906Luo, C, et al. 2014. GPC3 expression in mouse ovarian cancer induces GPC3‑specific T cell-mediated immune response through M1 macrophages and suppresses tumor growth. Oncology Reports, 913-921.
24554272Dohle, E, et al. 2014. Macrophage-mediated angiogenic activation of outgrowth endothelial cells in co-culture with primary osteoblasts. European cells and Materials, 149-164.
24270013Murata, T, et al. 2014. Differential susceptibility to hydrogen sulfide-induced apoptosis between PHLDA1-overexpressing oral cancer cell lines and oral keratinocytes: role of PHLDA1 as an apoptosis suppressor. Experimental Cell Research, 247-257.
24226264Hamada, S, et al. 2013. Thiamine deficiency induces massive cell death in the olfactory bulbs of mice. Journal of Neuropathology and Experimantal Neurology, 1193-1202.
24066047Müller, S, et al. 2013. Salt-dependent chemotaxis of macrophages. PLoS One, e73439.
23261461Tominaga, SI, et al. 2013. Presence of a novel exon 2E encoding a putative transmembrane protein in human IL-33 gene. Biochemical and biophysical research communications, 969-974.
21359963Okumura, F, et al. 2011. MDA-9/syntenin interacts with ubiquitin via a novel ubiquitin-binding motif. Molecular and cellular biochemistry, 163-172.

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