Quantitatively measure glutathione (GSH) and oxidized glutathione (GSSG) present in a variety of samples, including whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples. Analyze the absorbance colorimetric signal by spectrophotometer.
Glutathione, γ-L-glutamyl-L-cysteinyl-glycine or GSH, is the most abundant non-protein thiol in cells. GSH is involved in the regulation of a number of essential biochemical processes within the cell. Primarily recognized as a key intracellular source of reducing power for combating the toxic accumulation of free radical byproducts, GSH is also involved with detoxifying and removal of exo/endogenous toxins and alkylating agents. In its role as a cell signaling agent, GSH is involved in DNA synthesis and cell proliferation regulation. Due to the genetically conserved molecular processes by which cells die, intracellular levels of GSH can favor a cell death pathway via apoptosis (adequate intracellular GSH stores) or via necrosis or autophagy (depleted intracellular GSH stores). Detection of a drop in intracellular GSH concentration in an experimental cell population relative to a negative (healthy cell) control is often indicative of an apoptosis induction event. ICT’s Glutathione Colorimetric Detection Kit is designed to quantitatively measure GSH and GSSG in a variety of samples.
Glutathione and Oxidized Glutathione
Absorbance Plate Reader
Whole blood, serum, plasma, erythrocytes, urine, cell lysates, and tissue samples
2-8°C
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United States
- Prepare samples; treat with SSA to remove thiol-bearing protein.
- Prepare standard curve and blanks.
- If measuring oxidized GSH (GSSG), treat blanks, samples, and standards with 2VP.
- Add 50 µL blanks, standards, and samples to plate.
- Add 25 µL Colorimetric GSH Detection Substrate.
- Add 25 µL Reaction Mixture.
- Mix.
- Incubate 20 minutes at room temperature.
- Read at 405 nm.