Preserve activity of HRP conjugates and reduce background signal in immunoassays using HRP detection systems. HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques.
- Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil.
- Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 µL conjugate stock to 49.99 mL HRP Conjugate Stabilizer.
- Mix for 15 minutes.
- Use the diluted HRP conjugate in the immunoassay.
- Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.