Myeloperoxidase Detection Kit by Cell Technology

Myeloperoxidase (MPO) is a highly cationic glycosolated hemoprotein that has a molecular weight of 144kD. The hemoprotein consists of two dimmers linked via a disulfide bridge. Each dimmer is composed of a heavy (53kD) and light (15kD) sub-unit. Each heavy chain contains an independently acting protoporphyrin group containing a central iron (1-5). MPO is present in the azurophilic granules of polymorphonuclear leukocytes (PMNs) and is unique to neutrophils and monocytes. However, monocytes contain only one third of the MPO found in PMN’s. MPO utilizes H202 produced by the neutrophils to oxidize a varity of aromatic compounds to give substrate radicals for bactericidal activity (4 review). This enzyme is unique however in that it can oxidize chloride ions to produce a strong nonradical oxidant, HOCl. HOCl is the most powerfull bactericidal produced by neutrophils (4 review). Excessive production of these radicals can cause oxidative stress leading to oxidative tissue injury. The Fluoro MPO is a sensitive kit to detect myeloperoxidase (MPO) activity in neutrophils and macrophages as well as detection of PMN infiltration in tissue samples. The investigation of MPO has been important in the study of acute and chronic inflammatory disorders due to oxidative tissue damage and in acute and chronic manifestations of cardiovascular disease. The kit allows the monitoring of multiple time points to follow kinetics and is adaptable for high throughput screening.

530-571nm/590-600nm

Fluorescence plate reader

2-8 °C

Ships overnight (domestic), International Priority Shipping

United States