This mounting medium is made with limonene a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in Immunohistochemistry (IHC)
- Dehydrate tissue or cell smear slides with dehydrating agents e.g. 30 to 100% alcohol followed by xylene, toluene or any other dehydrating reagents.
- Add 2-3 drops of Organo mounting medium. Apply coverslip carefully, avoiding air bubbles.
- You are ready to visualize slide under microscope. The slides can be dried by heating at 50-60oC for 2-3 hours.