Mixing of reagent:
To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E), mix well. This ready to use reagent is good for 4-6 hours.
The unused solution can be discarded according to city, county, state, province or country’s regulations.
Application: IHC, ICC
- After the peroxidase reaction, wash slide with buffer 5-7X, (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/deionized water 2X.
- Add few drops of ready to use enhancing reagent for 1-2 minutes and discard. Washing of slide is not required.
- Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.