Pronase Reagent (Ready to Use)

Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.



SKU: AR-6542-01

Volume: 15ml
Price:
Sale price$70.25
Pronase is an antigen retriever solution used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Ready-to use Pronase reagent
2-8 °C; DO NOT FREEZE
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
  1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or ImmunoBioScience Universal Immuno buffer.
  2. Remove buffer, add 2-4 drops of Pronase reagent to cover tissue section.
  3. Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require 15 minutes at room temp).
  4. Discard Pronase reagent, wash 3-5 times with buffer.
  5. The tissue sections are ready for further IHC protocol.

Product Specific References

PMID Publication
22217002Kameyama, K., et al. 2012. Immunological characterization of Neospora caninum cyclophilin. Parasitology, 294-301.
21699673Awadin, W, et al. 2011. Detection of lumpy skin disease virus antigen and genomic DNA in formalin-fixed paraffin-embedded tissues from an Egyptian outbreak in 2006. Transboundary and Emerging Diseases, 451-457.

Bulk Order Pronase Reagent (Ready to Use)

Related Products

Recently viewed