Propidium Iodide (PI) is an intercalating fluorescent agent that binds between the bases of DNA. Propidium Iodide is membrane impermeant, which prevents DNA binding in viable cells, allowing identification of dead cells in a population.

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Propidium Iodide Stain
SKU: 638

Size: 1 mL
Sale price$22

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PI is an intercalating red fluorescent agent that binds between the bases of DNA. Upon binding to DNA, the fluorescence of Propidium Iodide (PI) is enhanced 20 to 30 fold. PI binding shifts the maximum fluorescence excitation by about 35 nm towards red while also shifting the maximum emission by about 15 nm towards blue. One molecule of PI stoichiometrically binds every four to five base pairs of DNA.
Necrotic and apoptotic cells
535 nm / 617 nm
Flow cytometry, Fluorescence microscope
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4.0 + 1.0
250 µg/mL
United States
  1. Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature.
  2. Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm.
  3. Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3.
  4. When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.

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Ventura, PMO;Gakovic, M;Fischer, BA;Spinelli, L;Rota, G;Pathak, S;Khameneh, HJ;Zenobi, A;Thomson, S;Birchmeier, W;Cantrell, DA;Guarda, G. Concomitant deletion of Ptpn6 and Ptpn11 in T cells fails to improve anticancer responses. EMBO reports. 2022 October 4; doi: 10.15252/embr.202255399. Abstract

Shu, L;Du, C. PHLDA1 promotes sevoflurane-induced pyroptosis of neuronal cells in developing rats through TRAF6-mediated activation of Rac1. Neurotoxicology. 2022 September 22; doi: 10.1016/j.neuro.2022.09.007. Article

Tian, J;Zhou, D;Xiang, L;Xie, B;Wang, B;Li, Y;Liu, X. Calycosin represses AIM2 inflammasome-mediated inflammation and pyroptosis to attenuate monosodium urate-induced gouty arthritis through NF-κB and p62-Keap1 pathways. Drug Development Research. 2022 September 7; doi: 10.1002/ddr.21985. Text

LaRock, DL;Johnson, AF;Wilde, S;Sands, JS;Monteiro, MP;LaRock, CN. Group A Streptococcus induces GSDMA-dependent pyroptosis in keratinocytes. Nature. 2022 May 1; doi: 10.1038/s41586-022-04717-x. Full Article

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