Protein Blocking Reagent (Animal Serum Free)

This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays. It does not contain azide therefore peroxidase is not inhibited. It does not contain any animal serum; it can be used for all IHC, ICC kits, ELISA and WB



SKU: AR-6581-02

Volume: 100 ml
Price:
Sale price$60.00
This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays. It does not contain azide therefore peroxidase is not inhibited. It does not contain any animal serum; it can be used for all IHC, ICC kits, ELISA and WB. Immunohistochemistry (IHC)/ Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products.
Buffer ready to use
Ships overnight (domestic), International Priority Shipping
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
United States
IHC/ICC procedure for frozen sections, paraffin sections and cell smears.
  1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol)
  2. Wash 2-3 with distilled or deionized water.
  3. Incubate sections/cell smear in Endoblocker (#1) for 5-10 minutes at room temperature or 37°C.
  4. Note: If antigen retriever is required it can be applied after this stage.
  5. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation Buffer IBSC cat # AR-6561) 3-5X.
  6. Incubate sections/ cell smear in Protein blocking solution, for 10 minutes. at RT or 37ºC
  7. Wash slide with PBS 1X.
  8. Follow instructions for IHC/ICC.

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