Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1X buffer solution at room temperature or 2-8oC for several weeks.
Application: Wash membrane or ELISA plates for 10 minutes each for 3 times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.